An UHPLC—MS instrument consisting on an Agilent 1290 extrem pressure liquid chromatographic system working in a flow range from 0.001 to 2.0 mL/min and achieving a maximum pressure of 100 MPa (15.000 Psi), coupled with a mass spectrometer API 3200 Applied Biosystems triple quadrupole working in a mass range from 5 to 1800 m/z and with a Turbo V source with TurboIonSpray and APCI probes. Software Analyst for API 3200/3200QTRAP.