GRUPO INSTITUTO NACIONAL DE INVESTIGACIÓN
Y TECNOLOGÍA AGRARIA Y ALIMENTARIA (INIA)
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GRUPO INSTITUTO NACIONAL DE INVESTIGACIÓN
Y TECNOLOGÍA AGRARIA Y ALIMENTARIA (INIA)
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Dirección Postal
Departamento de Tecnología de Alimentos, INIA, Ctra. de La Coruña
km 7, 28040-Madrid.
Pilar Gaya Sicilia. Investigador A2.
Email: pgaya@inia.es; tfno.: 91 3476762
Margarita Medina Fernández-Regatillo. Investigador A2.
Email: mmedina@inia.es; tfno.: 91 3476774
Manuel Nuñez Gutierrez. Investigador A1.
Email: nunez@inia.es; tfno.: 91 3476799
Eva Rodríguez Mínguez. Investigador A4.
Email: minguez@inia.es; tfno.:91 3474027
Sonia Garde López-Brea. Becaria postdoctoral.
Email: garde@inia.es tfno.:91 3474037
Juan Luis Arqués Orobón. Becario predoctoral.
Email: arques@inia.es; tfno.:91 3474027
Javier Tomillo Amor. Ayudante Técnico.
Email: tomillo@inia.es; tfno.:91 3474037
Líneas de
investigación
- Detección y caracterización de bacteriocinas de bacterias lácticas
- Tratamientos combinados de bacteriocinas con otros sistemas antimicrobianos.
- Utilización de bacterias lácticas productoras de bacteriocinas en la eliminación de microorganismos patógenos o alterantes de productos lácteos.
- Maduración dirigida de quesos mediante bacterias lácticas productoras de bacteriocinas.
Publicaciones del
grupo en el campo de las bacteriocinas
- Joosten H.M.L.J., Nuñez M. 1995. Adsorption of nisin and enterocin 4 to polypropylene and glass surfaces and its prevention by Tween 80. Letters in Applied Microbiology 21, 389-392.
- Joosten H.M.L.J., Gaya P., Nuñez M. 1995. Isolation of tyrosine decarboxylaseless mutants of a bacteriocin producing Enterococcus faecalis strain and their application in cheese. Journal of Food Protection 58, 1222-1226.
- Nuñez M., Tomillo J., Gaya P., Medina M. 1996. Bacteriocin quantification by the critical dilution method: a comparison of arbitrary units with diameter and area of zone of growth inhibition. Milchwissenschaf 51, 7-10.
- Joosten H.M.L.J., Nuñez M. 1996. Prevention of histamine formation in cheese by bacteriocin-producing lactic starter cultures. Applied and Environmental Microbiology 62, 1178-1181.
- Joosten H.M.L.J., Nuñez M., Devreese B., van Beeumen J., Marugg J.D. 1996. Purification and characterization of enterocin 4, a bacteriocin produced by Enterococcus faecalis INIA 4. Applied and Environmental Microbiology 62, 4220-4223.
- Rodríguez J.L., Gaya P., Medina M., Nuñez M. 1997. Bactericidal effect of enterocin 4 on Listeria monocytogenes in a model dairy system. Journal of Food Protection 60, 28-32.
- Nuñez M., Rodríguez J.L., García E., Gaya P., Medina M. 1997. Inhibition of Listeria monocytogenes by enterocin 4 during the manufacture and ripening of Manchego cheese. Journal of Applied Microbiology 83, 671-677.
- Garde S., Gaya P., Medina M., Nuñez M. 1997. Acceleration of flavour formation in cheese by a bacteriocin-producing adjunct lactic culture. Biotechnology Letters 19, 1011-1014.
- Joosten H.M.L.J., Rodríguez E., Nuñez M. 1997. PCR detection of sequences similar to the AS-48 structural gene in bacteriocin-producing enterococci. Letters in Applied Microbiology 24, 40-42.
- García E., De Paz M., Gaya P., Medina M., Nuñez M. 1997. Inhibition of Listeria innocua in Manchego cheese by bacteriocin-producing Enterococcus faecalis INIA 4. Milchwissenschaft 52, 667-670.
- Cogan T.M., Barbosa M., Beuvier E., Bianchi-Salvadori B., Cocconcelli P.S., Fernandes I, Gómez M.J., Gómez R., Kalantzopoulos G., Ledda A., Medina M., Rea M., Rodríguez E. 1997. Characterization of the lactic acid bacteria in artisanal dairy products. Journal of Dairy Research 64, 409-421.
- Rodríguez E., Tomillo J., Nuñez M., Medina M. 1997. Combined effect of bacteriocin-producing lactic acid bacteria and lactoperoxidase system activation on Listeria monocytogenes in refrigerated raw milk. Journal of Applied Microbiology 83, 389-395.
- Rodríguez E., Gaya P., Nuñez M., Medina M. 1998. Inhibitory activity of a nisin-producing starter culture on Listeria innocua in raw milk Manchego cheese. International Journal of Food Microbiology 39, 129-132.
- Tomillo J., Rodríguez E., Gaya P., Medina M., Nuñez M. 1998. Exposure to a commercial nisin preparation enhances Escherichia coli halotolerance. Milchwissenschaft 53, 69-73
- Zapico P., Medina M., Gaya P., Nuñez M. 1998. Synergistic effect of nisin and the lactoperoxidase system on Listeria monocytogenes in skim milk. International Journal of Food Microbiology 40, 35-42.
- Rodríguez E., Martínez M.I., Medina M., Hernández P., Rodríguez J.M. 1998. Detection of enterocin AS-48-producing dairy enterococci by dot-blot and colony hybridization. Journal of Dairy Research 65, 143-148.
- Martínez M.I., Rodríguez E., Medina M., Hernández P. E., Rodríguez J.M. 1998. Detection of specific bacteriocin-producing lactic acid bacteria by colony hybridization. Journal of Applied Microbiology 84, 1099-1103.
- Nuñez M., Zapico P., Medina M. 1998. Applications of bacteriocin-producing lactic cultures in dairy products. En: "Les bactéries lactiques - Lactic acid bacteria". Adria Normandie, Villers-Bocage, Francia, pp. 171-183.
- Zapico P., De Paz M., Medina M., Nuñez M. 1999 The effect of homogenization of whole milk, skim milk and milk fat on nisin activity against Listeria innocua. International Journal of Food Microbiology 46, 151-157.
- Oumer A., Garde S., Medina M., Nuñez M. 1999. Defined starter system including a bacteriocin producer for the enhacement of cheese flavour. Biotechnology Techniques 13, 267-270.
- Rodríguez E., González B., Gaya P., Nuñez M., Medina M. 2000. Diversity of bacteriocins produced by lactic acid bacteria isolated from raw milk. International Dairy Journal 10, 7-15.
- Rodríguez E., Arqués J.L., Gaya P., Tomillo J., Nuñez M., Medina M. 2000. Behaviour of Staphylococcus aureus in semi-hard cheese made from raw milk with a nisin-producing starter. Milchwissenschaft 55, 633-635.
- Garde S., Rodríguez E., Gaya P., Medina M., Nuñez M. 2001. PCR detection of the structural genes of nisin Z and lacticin 481 in Lactococcus lactis subsp. lactis INIA 415, a strain isolated from raw milk Manchego cheese. Biotechnology Letters 23, 85-89.
- Oumer A., Gaya P., Fernández E., Mariaca R., Garde S., Medina M., Nuñez M. 2001. Proteolysis and formation of volatile compounds in cheese manufactured with a bacteriocin-producing adjunct culture. Journal of Dairy Research 68, 117-129
- Rodríguez E., Arqués J.L., Gaya P., Nuñez M., Medina M. 2001. Control of Listeria monocytogenes by bacteriocins and monitoring of bacteriocin-producing lactic acid bacteria by colony hybridation in semi-hard raw milk cheese. Journal of Dairy Research 68, 131-137.
- Oumer A., Garde S., Gaya P., Medina M., Nuñez M. 2001. The effects of cultivating lactic starter cultures with bacteriocin-producing lactic acid bacteria. Journal of Food Protection 64, 81-86.
- Tratamientos combinados de bacteriocinas y otros sistemas inhibitorios para la mejora de la seguridad de los productos lácteos. Juan Luis Arqués Orobón.
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