Study paves way for healthier and more robust eggs


A mound of different coloured eggs


The results of a study recently published in the prestigious international journal Science Advances have enabled researchers to better understand the role of eggshells in embryo development and hatching. The objective of the study, conducted by an international research team led by Marc McKee from McGill University in Canada and involving the participation of scientists from the University of Granada (UGR), was to analyse the nanostructure of chicken eggshells. The findings could be used to produce healthier, more robust eggs by providing researchers with the means to genetically select laying hens with specific characteristics.

An eggshell is made up of both organic and inorganic matter that contains calcium carbonate. One of the important findings of the study was that the nanostructure was closely linked to the presence of osteopontin, a protein which is also found in bones.

Eggshell transformation process

Eggshells are strong enough to resist fractures during the incubation period. However, they gradually weaken as the hatching period approaches to make it easier for the chicks to break through the shell. The eggshell weakens as its internal layer dissolves, releasing calcium which, in turn, is needed by the embryo for bone formation. The study found that this process is made possible as a result of the changes that occur in the eggshell nanostructure during the incubation period.

Implications for food safety

Furthermore, the researchers were able to recreate similar nanostructures to those they discovered in the eggshells by using proteins, specifically by adding osteopontin to mineral crystals grown in the lab. The team add that: “A better understanding of the role of proteins in the calcification process that strengthens the eggshell structure could have significant implications for food safety.” According to the team, which includes Alejandro B. Rodríguez Navarro from the Department of Mineralogy and Petrology (UGR), approximately 10 per cent of all eggs break or crack before consumption, which increases the risk of food poisoning and infections such as Salmonella. Understanding how the different mineral nanostructures contribute to strengthening the eggshell could allow scientists to genetically select laying hens based on specific traits, which would put healthier, more resistant eggs into circulation.

However, studying the internal structure of eggshells can be challenging because of the ease with which they break when under analysis. To overcome this obstacle, the team used a focused ion beam sectioning system that allowed them to accurately cut the samples out of the eggshells and study them using electron microscopy.

The full paper is available via this link:

Athanasiadou et al. Nanostructure, osteopontin, and mechanical properties of calcitic avian eggshell. Science Advances 30 Mar 2018: Vol. 4, no. 3, eaar3219 DOI: 10.1126/sciadv.aar3219


Two researchers sitting in a laboratory surrounded by computer screens and machines


Contact information:

Alejandro B. Rodríguez Navarro

Department of Mineralogy and Petrology (UGR)